Biochemistry and Biophysics (BAB)

Editor-in-Chief: Prof. Hara P. Misra
Frequency: Continuous Publication
ISSN Online: 2328-1642
ISSN Print: 2328-1693
Paper Infomation

Age-Dependent Increase in Ca2+ Exchange Magnetosensitivity in Rat Heart Muscles

Full Text(PDF, 572KB)

Author: Lilia Y. Narinyan, Gayane S. Ayrapetyan, Jaysankar De, Sinerik N. Ayrapetyan

Abstract: Previously the higher magnetosensitivity of Na+/K+ pump-α3 isoforms and its age-dependent dysfunction were shown. It was suggested that the latter could be a consequence of inhibition of Ca2+ efflux from the cell. To check this suggestion, the age-dependency of 45Ca2+ exchange, and their ouabain- and magnetosensitivities in rat heart muscles were studied. The initial rate of 45Ca2+ exchange in muscles of young rats was significantly higher than in older ones. Intraperitoneal injections of 10-9 M ouabain led to activation of 45Ca2+ uptake as a result of its absorption by intracellular structure that had age-dependent weakening character. The static magnetic field (SMF) exposure on ouabain-poisoned rats had inhibitory effect on young and activation on older. The rate of 45Ca2+ efflux in ouabain non-poisoned heart muscles had age-dependent weakening character but its magnetosensitivity increased. The 10-9 M ouabain had activation, while at 10-4 M concentration it had inhibition effects on 45Ca2+ efflux in young rats. Nevertheless, in older rats, both concentrations of ouabain had activation effect on 45Ca2+ efflux. The SMF exposure had age-dependent activation effect on 45Ca2+ efflux in tissues bathing in physiological (PS) and in ouabain solutions. The SMF-induced activation of 45Ca2+ efflux was more expressed in tissues of older rats poisoned by 10-4 M ouabain. We suggest that the age-dependent depression in capacity of [Ca2+]i buffer system should be the result of increase in [Ca2+]i due to dysfunction of Na+/K+ pump is responsible for age-dependent increase in magnetosensitivity of 45Ca2+ exchange in heart muscles.

Keywords: Na+/K+ Pump, Na+/Ca2+ Exchange, Age, Heart Muscle, Ca2+ Pump


[1] AC-adenylyl cyclase, GC-guanylate cyclase, Rr-ryanodine receptors, CaM-calmodulin, cAMP-cyclic adenosine monophosphate, cGMP-cyclic guanosine monophosphate.

[2] Adams HR, Parker JL, Mathew BP. The influence of ketamine on inotropic and chronotropic responsiveness of heart muscle. J Pharmacol Exp Ther 201(1), 1977:171-183.

[3] Adey WR. Tissue interactions with non-ionizing electromagnetic field. Physiol Rev 61(1981):435-514.

[4] Adrian RH. The effect of internal and external K concentration on the membrane potential of frog muscle. J Physiol (Lond) 133 (1956):631–658.

[5] Akbar L, Sarioğlu Y, Utkan T. Effect of ketamine on contractile performance of isolated frog myocardium and comparison of ketamine, thiopental and droperidol. Mater Med Pol 24(1), 1992:32-34.

[6] Ayrapetyan G, Papanyan A, Hayrapetyan H, Ayrapetyan S.Metabolic pathway of magnetized fluid-induced relaxation effects on heart muscle. Bioelectromagnetics 26(8), 2005:624-630.

[7] Ayrapetyan SN, Rychkov GY, Suleymanyan MA. Effects of water flow on transmembrane ionic currents in neurons of Helix pomatia and in Squid giant axon. Comp. Biochem Physiol 89A (1988):179-186.

[8] Ayrapetyan SN, Suleymanyan MA, Saghyan AA, Dadalyan SS. Autoregulation of the selectrogenic Na pump. Cell Mol Neurobiol 4(1984):367-384.

[9] Ayrapetyan SN. Cell hydration as a universal marker for detection of environmental pollution. The Environmentalist 32(2), 2012:210-221.

[10] Azatian K, White A, Walker R, Ayrapetyan S. Cellular and molecular mechanisms of nitric oxide-induced heart muscle relaxation. Gen Pharmacol 30(4), 1998:543-553.

[11] Baker PF, Blaustein MP, Hodgkin AL, Steinhardt SA. The influence of Ca on Na efflux in squid axons. J Physiol 200 (1969): 431-458.

[12] Blackman CF, Benane SG, Elliott DJ, House DE, Pollock MM. Influence of electromagnetic fields on the efflux of calcium ions from brain tissue in vitro: a three-model analysis consisted with the frequency response up to 510 Hz. Bioelectromagnetics 9(3), 1988:215–227.

[13] Blaustein MP, Lederer WJ. Na+/Ca2+ exchange. Its physiological implications. Physiol Rev 79(1999):763-854.

[14] Blaustein MP, Zhang J, Chen L, Song H, Raina H, Kinsey SP, Izuka M, Iwamoto T , Kotlikoff MI, Lingrel JB, Philipson KD, Wier WG, Hamlyn JM. The Pump, the Exchanger, and Endogenous Ouabain: signaling mechanisms that link salt retention to hypertension. Hypertension 53(2009):291-298.

[15] Brini M, Carafoli E. Calcium Pumps in Health and Disease. Physiol Rev 89(4), 2009:1341-1378.

[16] Danielyan AA, Mirakyan MM, Grigoryan GY, Ayrapetian SN. The Static Magnetic Field on Ouabain H3 Binding by Cancer Tissue. Physiol Chem Phys Med NMR 31(2), 1999:139-144.

[17] Dipolo R, Beaugé L. Na+/Ca2+ Exchanger: Influence of metabolic regulation on ion carrier interaction. Physiol Rev 86(2006):155-203.

[18] Hodgkin A. The conduction of the nervous impulse. Liverpool: Liverpool University Press, 1964.

[19] Juhaszova M, Blaustein M. Na+ pump low and high ouabain affinity alpha subunit isoforms are differently distributed in cells. Proc Natl Acad Sci USA 94(5), 1997:1800-1805.

[20] Khachaturian ZS. . The role of calcium regulation in brain aging: reexamination of a hypothesis. Aging 1(1989):17–34.

[21] Kostyuk P, Lukyanetz E.Intracellular calcium signaling-basic mechanisms and possible alterations In: Bioelectromagnetics: Current Concepts. Eds. Ayrapetyan S and Markov M, NATO Science Series, Dordrecht (Netherland): Springer Press, 2006, p 87-122.

[22] Liu J, Tian J, Haas M, Shapiro J, Askari A, Xie Z.. Ouabain Interaction with Cardiac Na+,K+-ATPase Initiates Signal Cascades Independent of Changes in Intracellular Na+ and Ca2+ Concentrations. J Biol Chem 275(36), 2000:27838-27844.

[23] Narinyan L, Ayrapetyan G, Ayrapetyan S.Age-dependent magnetosensitivity of heart muscle hydration. Bioelectromagnetics 33(2012): 452-458.

[24] Narinyan L, Ayrapetyan G, Ayrapetyan SAge-dependent magnetosensitivity of heart muscle ouabain receptors. Bioelectromagnetics 34(4), 2013:312-322.

[25] Parsegian VA, Rand RP, Rau DC. Osmotic stress, crowding, preferential hydration, and 3 binding: a comparison of perspectives. Proc Natl Acad Sci USA 97(2000):3987-3992.

[26] Pollack G.. In: Cells, Gels and the Engines of Life. Ebner & sons, Seatle, WA,USA, 2008,p170- 178.

[27] Sagian AA, Ayrapetyan SN, Carpenter DO. Low dose of ouabain stimulates the Na:Ca exchange in Helix neurons. Cell Mol Neurobiol 16(1996):180-192.

[28] Siegel GJ. Basic Neurochemistry, 6th edition: Molecular, Cellular and Medical Aspects Philadelphia: Lippincott-Raven, 1999, p1183.

[29] Takahashi R, Aprison M. Acetylcholine content of discrete areas of the brain obtained by a near-freezing method. J Neurochem 11 (1964):887-892.