Biochemistry and Biophysics (BAB)

Editor-in-Chief: Prof. Hara P. Misra
Frequency: Continuous Publication
ISSN Online: 2328-1642
ISSN Print: 2328-1693
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Age-Dependent Increase in Ca2+ Exchange Magnetosensitivity in Rat Heart Muscles

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Author: Lilia Y. Narinyan, Gayane S. Ayrapetyan, Jaysankar De, Sinerik N. Ayrapetyan

Abstract: Previously the higher magnetosensitivity of Na+/K+ pump-α3 isoforms and its age-dependent dysfunction were shown. It was suggested that the latter could be a consequence of inhibition of Ca2+ efflux from the cell. To check this suggestion, the age-dependency of 45Ca2+ exchange, and their ouabain- and magnetosensitivities in rat heart muscles were studied. The initial rate of 45Ca2+ exchange in muscles of young rats was significantly higher than in older ones. Intraperitoneal injections of 10-9 M ouabain led to activation of 45Ca2+ uptake as a result of its absorption by intracellular structure that had age-dependent weakening character. The static magnetic field (SMF) exposure on ouabain-poisoned rats had inhibitory effect on young and activation on older. The rate of 45Ca2+ efflux in ouabain non-poisoned heart muscles had age-dependent weakening character but its magnetosensitivity increased. The 10-9 M ouabain had activation, while at 10-4 M concentration it had inhibition effects on 45Ca2+ efflux in young rats. Nevertheless, in older rats, both concentrations of ouabain had activation effect on 45Ca2+ efflux. The SMF exposure had age-dependent activation effect on 45Ca2+ efflux in tissues bathing in physiological (PS) and in ouabain solutions. The SMF-induced activation of 45Ca2+ efflux was more expressed in tissues of older rats poisoned by 10-4 M ouabain. We suggest that the age-dependent depression in capacity of [Ca2+]i buffer system should be the result of increase in [Ca2+]i due to dysfunction of Na+/K+ pump is responsible for age-dependent increase in magnetosensitivity of 45Ca2+ exchange in heart muscles.

Keywords: Na+/K+ Pump, Na+/Ca2+ Exchange, Age, Heart Muscle, Ca2+ Pump



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