International Journal of Environment and Resource (IJER)

Editor-in-Chief: Prof. Domenico Pianese
Frequency: Continuous Publication
ISSN Online: 2324-7029
ISSN Print: 2324-6995
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Characterization of E. coli Strains Obtained from Wastewater Effluent

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Author: Grażyna A. PŁAZA, Agnieszka TUREK, Renata SZCZYGŁOWSKA

Abstract: The aim of the study was the characterization of E. coli isolates obtained from wastewater effluent. The following features were evaluated: enzymatic profile by API ZYM test, antibiotic resistance, biocide susceptibility, and hydrophobicity by BAH and SAT methods. The similar enzymatic pattern was obtained for all number of E. coli. Among the tested isolates O157:H7 E. coli were no identified. E. coli isolates were tested regarding their resistance to 20 different antibiotics. 90% of E. coli isolated were categorized as resistant. The highest resistance frequencies were found for the following antibiotics: cephalotin and erythromycin (90%), nitrofuzantoin (94%), rifampicin (97%) and novobiocin (100%). The collection of bacterial strains was also analyzed for multiple antibiotic resistance (MAR). The data indicated that 88% of bacteria showed resistance to 8 - 15 antibiotics. 3 % of isolates were resistance to 19 tested antibiotics. No strain was detected to be resistance from 0 to 5 antibiotics. The decrease of susceptibility of bacterial isolates to antibiotics was probably caused by the presence of these compounds in the wastewaters and the long exposition of E. coli strains to them. The isolates were susceptibility to tested biocides. Among the Preventol® biocides, the D2 type was the most active. The similar activity showed LV508 type from the Acticide® biocides. Only 17% of the isolates revealed moderate hydrophobicity.

Keywords: E. Coli Strains, Antibiotics, Biocides, Hydrophobicity



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